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shRNA对胃癌细胞株SGC-7901 VEGF-C表达的抑制作用

Inhibitory Effect of shRNA on Expression of the VEGF-C in SGC-7901 Cells
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摘要 目的:筛选出有效干扰胃癌SGC-7901细胞株血管内皮生长因子-C(vascular endothelial growth factor C,VEGF-C)的短发卡状RNA(short hairpin RNA,shRNA)。方法:设计2个shRNA片段,VEGFC-1505与VEG-FC-1417,体外转染VEGF-C阳性表达的胃癌细胞株SGC-7901,采用RT-PCR检测VEGF-C mRNA表达水平的变化,western-blot检测VEGF-C蛋白水平变化。结果:VEGFC-1505可以显著抑制VEGF-C基因表达,对其mRNA表达的抑制率可以达到53%,对蛋白的表达抑制率可以达到92.9%。结论:成功筛选出针对胃癌SGC-7901细胞株VEGF-C的shRNA,为抑制肿瘤淋巴管生成和转移研究提供有效的实验工具。 Objective:To screen short hairpin RNA(shRNA)that can interfere with VEGF-C of stomach cancer cell line SGC-7901 effectively.Methods:Two shRNA fragments(VEGFC-1505 and VEGFC-1417)were designed and transfected into stomach cancer cell line SGC-7901 that express VEGF-C positively.The mRNA expression level of VEGF-C was detected with RT-PCR,and the protein of VEGF-C was detected with western-blot.Results:VEGFC-1505 could depress the expression of VEGF-C dramatically,and the inhibition rate of the VEGF-C mRNA re...
作者 达明绪 罗婷 赵紫罡 王捷
机构地区 中山大学附属第二医院普外科 四川大学华西医院 包头市肿瘤医院外二科
出处 《华西医学》 CAS 2008年第5期1083-1086,共4页 West China Medical Journal
基金 广东省自然科学基金No.07301381 中国博士后科学基金No.20060400777
关键词 RNA干扰技术 胃癌SGC-7901细胞株 VEGF-C RNA interference stomach cell line SGC-7901 VEGF-C
分类号 R735.2 [医药卫生—肿瘤]
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参考文献10

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华西医学
2008年 第5期

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