胡枝子属植物ITS序列研究与系统发育分析

Phylogenetic Relationship of Genus Lespedeza by ITS Sequence Data

用PCR产物直接测序法对10种胡枝子属植物和1个外类群的ITS序列进行了测定。结果表明,10种胡枝子ITS序列全长在612～622bp之间,其中ITS1长度在243～228bp之间,变异较大,ITS2长度在215～220bp,5.8S的长度均为165bp,比较保守。序列比对结果发现有76个变异位点,占比对序列长度的11.93%。构建了胡枝子属植物ITS序列的系统发育树,并探讨了参试胡枝子种质的亲缘关系。

ITS sequences of ten kinds of 10s plants of Lespedeza and an outgroup were obtained by primer design PCR,sequencing and cluster analysis.The results showed that ITS1 section length was 228~243 bp 5.8S sequence length was 165 bp which was too conservative,and the ITS2 section length varied from 215 bp to 220 bp and the conservative sites occupied 88.1%.Phylogenetic analysis of ITS sequences using PAUP 4.0 software and their genetic relationship was discussed