八仙花茎段组织培养技术研究

Tissue Culture of Stem Segment of Hydrangea macrophylla

以八仙花带腋芽茎段为外植体,研究了其组织培养技术。结果表明:以70%酒精处理30 s+0.1%升汞处理10 min,成活率可达96%;以MS+6-BA0.5 mg/L+IBA0.5 mg/L为启动培养基,诱导率可达100%;以MS+6-BA0.5 mg/L+IBA0.3 mg/L为增殖培养基,培养25 d,增殖系数可达9.2;以1/2MS+IBA0.3 mg/L进行生根培养,生根率100%,根长势良好且根毛区长达1.1cm;移栽到以珍珠岩∶腐殖土=1∶2的基质中,覆盖地膜保湿7 d,成活率可达80%以上。

Stems with axillaries bud of Hydrangea macrophylla were used as explants to conduce in vitro culture.The results showed that the optimum disinfector was 70% alcohol for 30 seconds and HgCl2 for 10 minutes.The induction culture medium was MS+BA0.5 mg/L+IBA0.5 mg/L and the ratio of induction was 100%.The optimum multiplication culture medium was MS+BA0.5 mg/L+IBA 0.3 mg/L and after 25 days multiplication coefficient was 9.2.The rooting culture medium was 1/2MS+IBA 0.3 mg/L and the ratio of rooting was 100%,an...