绿茶对苯并芘诱发大鼠肺癌的预防及其机制初探

Prevention of chinese green tea on 3,4-benzopyrene-induced lung cancer and its mechanism in animal mode

背景和目的中国绿茶为人们喜爱的饮料,含有多种抑制肿瘤细胞生长的茶多酚,有可能成为有效的防癌饮料。本实验探讨绿茶对苯并芘诱发大鼠肺癌的预防作用并初步探讨其机制。方法对3组雌性SD大鼠采用相同的饲料及不同的饮料饲养,A组仅饮绿茶,B、C组仅饮水。3组大鼠均接受4次肺内注射处理:A、B组均注射苯并芘-玉米油混合物,C组注射玉米油。1年后处死全部仍存活大鼠。取肿块及全部大鼠的肺组织进行HE染色病检。并比较各组大鼠肺癌p53、Bcl-2表达。结果观察1年C组无肺癌发生,B组大鼠发生肺癌15例,成瘤率达75%(15/20),A组大鼠发生肺癌6例,成瘤率为30%(6/20),A、B两组之间成瘤率差异有统计学意义(P<0.01)。绿茶干预组肺癌组织p53基因mRNA及蛋白水平表达均有上调趋势,但差异无统计学意义(P>0.05),Bcl-2基因mRNA及蛋白水平表达则均明显下调(P<0.05)。结论绿茶对肺癌具有明显的预防作用;绿茶对p53表达具有一定的上调作用,但下调Bcl-2表达的作用更明显,这可能与其防癌机制有关。

Background and objective Chinese green tea is one of the daily consumption beverages in the world and is considered a promising cancer chemopreventive agent. In the present study, we investigate the role of lung cancer prevention by green tea and its mechanism. Methods Three groups of female SD rats were kept with the same feed. Rats in group A were administrated with 1% green tea drinking, while in group B and group C with water only. Animals in group A and group B were given 3,4-benzopyrene-corn oil mixtu...