清开灵注射液对大鼠阳离子化牛血清白蛋白肾炎的作用机制研究

Mechanism of Qingkailing Injection on Nephritis Induced by Cationic Bovine Serum Albumin in Rats

目的:探讨清开灵注射液对大鼠阳离子化牛血清白蛋白(C-BSA)肾炎的作用机制。方法:采用C-BSA建立肾小球肾炎大鼠模型,治疗用清开灵注射液腹腔注射,标本取大鼠肾脏皮质和全血,分别做NF-кB表达,IL-2、IL-6和TNF-α含量测定。结果:与正常对照组相比,模型组的NF-кB表达显著增高(P<0.01),IL-2、IL-6和TNF-α含量显著增高(P<0.05);与模型组相比,清开灵治疗组NF-кB表达,IL-2、IL-6和TNF-α含量则明显降低(P<0.05)。结论:清开灵注射液通过抑制NF-кB的活化,降低炎症免疫细胞因子表达,起到治疗肾小球肾炎的作用,因而从分子水平揭示了肾炎的免疫发病机制和清热解毒药物治疗肾小球肾炎的部分机制。

Objective: To study the mechanism of qingkailing injection on nephritis induced by cationic bovine serum albumin in rats.Methods: C-BSA induced nephritis model was utilized in rats.The mechanism of qingkailing injection on this model was investigated.Results: The expression of NF-κB and the concentration of IL-2,IL-6 and TNF-α were significantly increased in model group(P<0.01~0.05);whereas qingkailing group was decreased significantly(P<0.05).Conclusions: This study clearly suggests that qingkailing ...