山苍子根煎剂对CIA大鼠血清TNF-α,IL-1 β水平影响

Effect of Litsea cubeba Root Decoction on the Serum Levels of TNF-α,IL-1 β in Rats with Collagen Ⅱ -induced Arthritis(CIA)

目的观察山苍子根对CIA大鼠关节炎指数、血清TNF-α,IL-1 β水平的影响,探讨其对类风湿关节炎可能的疗效机制。方法用鸡Ⅱ型胶原蛋白诱导CIA大鼠模型,记录各组大鼠的关节炎指数(AI);ELISA检测血清TNF-α及IL-1 β水平。结果造模大鼠68.2%出现关节炎症状(雄鼠52.7%,雌鼠83.6%);给药24d后,山苍子根中、高剂量组AI较模型对照组明显降低(P<0.01);模型对照组、雷公藤多甙组、强的松组、山苍子根各组血清TNF-α及IL-1 β水平较正常对照明显增高(P均<0.01);与模型对照组比较雷公藤多甙组、强的松组、山苍子根各组血清TNF-α及IL-1 β水平明显下降(P均<0.01),且IL-1 β下降水平随山苍子根剂量的增加而增加。结论山苍子根可缓解CIA大鼠关节肿胀,抑制CIA大鼠血清TNF-α及IL-1 β水平,且随山苍子根剂量的增加其下调血清IL-1 β水平的作用亦随之增加。山苍子根治疗RA的机制可能与其下调血清TNF-α和IL-1 β水平有关。

Objective To investigate the effect of Litsea cubeba root decoction on the serum levels of TNF-α,IL-1 βin rats with collagen Ⅱ-induced arthritis(CIA),and explore its related immunological mechanisms.Method The rat experimented arthritis model was established by subcutaneous injection with collagen Ⅱ from chicken; Rats were distributed into seven groups randomly:normal control group,model control group,Tripterygium glycosides group,prednisone group,low-,middle-and high-dosage Litsea cubeba groups;The severit...