MSP技术检测慢性淋巴细胞白血病患者孕激素受体B基因甲基化异常

Detecting the Aberrant Methylation of Progesterone Receptor B Gene in Human Chronic Lymphocytic Leukemia by MSP

目的建立检测慢性淋巴细胞白血病患者孕激素受体B基因CpG岛甲基化状态改变的方法。方法提取外周血DNA,经亚硫酸盐修饰,然后采用甲基化特异性PCR技术检测9例慢性淋巴细胞白血病患者及5例正常对照外周血DNA孕激素受体B基因CpG岛甲基化状态。结果在9例慢性淋巴细胞白血病患者外周血中5例成功地检测到孕激素受体B基因甲基化异常,而正常对照均没有发现甲基化异常。结论建立的检测慢淋孕激素受体B基因异常甲基化的方法是可靠的,该方法可能对监测肿瘤进程有一定作用。

Objective To set up a method detecting the methylation status of progesterone receptor B gene using peripheral blood from chronic lymphocytic leukemia patients.Methods DNA was isolated from peripheral blood and treated with Sodium Bisulfite,then analysed by methylation-specific polymerase chain reaction(MSP) method. Nine samples from patients and five controls were analysed.Results PR-B gene showed aberrant methy-lations in 5 samples from patients,but none in normal controls.Conclusion We set up a reliable ...