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Th1/Th2细胞因子平衡偏移对巨噬细胞FIZZ1表达的影响 被引量：4

Effect of Th1/Th2 Cytokine Balance Changing on the Expression of Macrophage FIZZ1
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摘要 目的探讨Th1/Th2细胞因子平衡动态改变对巨噬细胞FIZZ1表达的影响。方法收集小鼠腹腔巨噬细胞,培养后将贴壁细胞分为6组,分别用不同比例的重组INFγ+重组IL-4刺激培养的巨噬细胞,RT-PCR检测巨噬细胞内FIZZ1mRNA表达,荧光免疫组化激光共聚焦显微镜检测FIZZ1蛋白表达。结果Th2型细胞因子IL-4刺激巨噬细胞,FIZZ1mRNA及其蛋白明显表达,INF-γ拮抗IL-4引起的巨噬细胞FIZZ1mRNA及其蛋白表达(INFγ+IL-4组与IL-4组比较,P<0.05),模拟Th1/Th2细胞因子平衡偏向Th2时,FIZZ1mRNA及其蛋白表达增加。结论Th2型细胞因子IL-4能刺激巨噬细胞表达FIZZ1,Th1型细胞因子INF-γ拮抗IL-4引起的巨噬细胞FIZZ1mRNA及其蛋白表达,Th1/Th2细胞因子平衡偏向Th2时,巨噬细胞FIZZ1表达增加。 Objective To explore the effect of Th1/Th2 cytokine balance switching on macrophage FIZZ1expression.Methods Collected mouse peritoneal macrophages,cultured macrophages were stimulated by different dose of recombinant INFγ+ and recombinant IL-4,adherent cells were divided into 6 groups:control groups;recombinant INFγ groups;recombinant IL-4 groups and recombinant INFγ+ recombinant IL-4 groups. FIZZ1 mRNA and protein expressions of macrophages were detected by RT-PCR and laser confocal microscope. Results Whe...
作者 张红明 何作云 李晓燕
机构地区 济南军区总医院心内科 第三军医大学新桥医院心内科
出处 《中国血液流变学杂志》 CAS 2008年第1期17-20,共4页 Chinese Journal of Hemorheology
关键词 FIZZ1 TH细胞 细胞因子 巨噬细胞 FIZZ1 Helper T lymphocyte Cytokine Macrophage
分类号 R363 [医药卫生—病理学]
  • 相关文献

参考文献9

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共引文献65

同被引文献64

  • 1李晓燕,房洁,张红明,韩淑芳,高玉琪,晋群.FIZZ1/RELMα对大鼠主动脉内皮细胞血管新生的影响及其机制探讨[J].中华临床医师杂志（电子版）,2012,6(21):6713-6717. 被引量：1
  • 2马万里,叶红,陶晓南,辛建保.实验性肺纤维化大鼠发病过程中肺组织FIZZ1蛋白和mRNA表达的动态变化[J].生理学报,2005,57(4):493-497. 被引量：10
  • 3叶红,马万里,张少华,徐清,付世欧,刘声远,王迪浔.慢性吸烟大鼠肺组织FIZZ1/REL Mα表达的变化[J].中国病理生理杂志,2006,22(5):938-942. 被引量：5
  • 4Holcomb IN, Kabakoff RC, Chan B, et al. FIZZ1, a novel cysteine-rich secreted protein associated with pulmonary inflammation, defines a new gene family. EMBO J,2000,19 4046-4055. T.
  • 5eng X, Li D, Champion HC, et al. FIZZ1/RELMalpha, a novel hypoxia-induced mitogcnic factor in lung with vasoconstrictive and angiogenic properties. Circ Res, 2003, 92:1065-1067.
  • 6He W, Wang ML, Jiang HQ, et al. Bacterial colonization leads to the colonic secretion of RELMheta/FIZZ2, a novel goblet cell specific protein. Gastroenterology,2003,125:1388- 1397.
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中国血液流变学杂志
2008年 第1期

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