红叶石楠愈伤组织抗褐化研究以及悬浮细胞系的建立

Studies on the decreasing browning of callus and the establishment of suspension cell lines from Photinia frasery

以红叶石楠无菌苗叶片为试材,诱导愈伤组织并建立悬浮细胞系。结果表明:红叶石楠愈伤组织初代诱导最佳培养基为MS+6-BA 0.5 mg/L+2,4-D 1.0 mg/L+NAA 0.1 mg/L,诱导率可达97%。继代培养以MS+6-BA 1.0 mg/L+NAA 0.1 mg/L为宜。针对红叶石楠愈伤组织继代培养过程中易褐化的问题,通过正交试验探讨了不同吸附剂、抗氧化剂及其它因素对褐化的影响。采用MS+6-BA 1.0 mg/L+NAA 0.2 mg/L+Vc 1.0 mg/L+Ch 100 mg/L附加1%蔗糖的继代培养基,可以明显降低红叶石楠愈伤组织的褐化现象。将胚性愈伤组织接种到1/2 MS+6-BA 1.0 mg/L+NAA 0.1 mg/L附加2%蔗糖,pH 5.5液体培养基中悬浮震荡培养获得了分散性好、色泽鲜艳的悬浮细胞。

Asepsis leaves of Photinia frasery were employed to study callus tissue culture and establishment of suspension cell lines.The results showed that the appropriate medium for callus induction is MS+6-BA 0.5 mg/L +2,4-D 1.0 mg/L+NAA 0.1 mg/L;Inductivity is up to 97%;And the medium of multiplication is MS+6-BA 1.0 mg/L+NAA 0.1 mg/L.Orthogonal tests were performed to find out the effects of different adsorbents,anti-oxidants and other factors on callus browning.The medium for anti-browning is MS+6-BA 1.0 mg/L +...