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巢式PCR与免疫组化在检测肾癌组织支原体中的应用 被引量：1

Application of nested PCR and immunohistochemistry in the detection of mycoplasma in renal cell carcinoma
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摘要 目的:评价巢式PCR和免疫组化技术在检测肾癌组织支原体中的应用和价值。方法:采用支原体通用引物巢式PCR技术和单克隆抗体PD4免疫组化技术,检测95例肾癌组织中支原体DNA和支原体p40蛋白表达。结果:免疫组化检测和巢式PCR检测的阳性检出率分别为64.2%和81.1%,巢式PCR技术检测肾癌组织支原体在灵敏度、特异度、约登指数、阳性预测值和阴性预测值方面均优于免疫组化技术。结论:巢式PCR和免疫组化技术均可作为肾癌组织支原体的检测方法,巢式PCR较免疫组化检测敏感。 Objective:To investigate the comparison and estimation of nested PCR and Immunohistochemistry (IHC) in the detection of mycoplasma in renal cell carcinoma (RCC). Methods: There were 95 samples of archived embedded tissues of RCC. Mycoplasma DNA and mycoplasma p40 in RCC were detected by nested PCR with mycoplasma universal primer and by IHC with mycoplasma monoclonal antibody (MAb) PD4 respectively. Results: The positive detection ratio of IHC and nested PCR were 64.2% and 81.1%, respectively. It was showed...
作者 曹军 李建平
机构地区 西安交通大学医学院第二附属医院泌尿外科
出处 《陕西医学杂志》 CAS 北大核心 2008年第9期1216-1218,1221,共4页 Shaanxi Medical Journal
基金 陕西省科学技术研究发展计划项目(No2008k09-04)
关键词 肾肿瘤/诊断 支原体感染/诊断 聚合酶链反应 免疫组织化学 对比研究 Kidney neoplasms/diagnosis Mycoplasmas infections/diagnosis Polymerase chain reaction Immunohistochemistry Comparative study
分类号 R737.11 [医药卫生—肿瘤]
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参考文献11

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二级参考文献54

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共引文献29

同被引文献9

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引证文献1

二级引证文献3

陕西医学杂志
2008年 第9期

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