雌激素对小鼠参考记忆的影响研究

Effects study of estrogen replacement on reference memory in mice

目的:探讨雌激素去除(OVX)与雌激素替代(ER)对小鼠参考记忆的影响。方法:将3月龄的雌性C57BL/6J小鼠40只随机分为对照组和卵巢切除组,卵巢切除组又分为不给雌激素、给雌激素7d、给雌激素40d的3个亚组,每组10只。小鼠摘除卵巢后饲养至7月龄进行8臂迷宫实验,将给雌激素组小鼠于迷宫测试前7d和40d分别皮下埋植控释雌激素(17β-雌二醇)药丸(0.18mg/丸),测试各组小鼠在8臂迷宫的参考记忆错误率。结果:卵巢切除组不给雌激素亚组其参考记忆错误率明显高于对照组(P<0.05);给雌激素7d和40d亚组其参考记忆错误率明显下降(P<0.05,P<0.01),而且长期给雌激素亚组其参考记忆错误率明显少于短期给雌激素亚组。对照组和给雌激素组其错误率无显著性差异(P>0.05)。结论:雌激素去除明显损害了小鼠的空间参考记忆,而雌激素替代可以改善小鼠的记忆能力,并且长期的替代改善效果更明显。

Obejective:To investigate the effects of ovariectomy and estrogen replacement on spatial reference memory in mice using radial arm maze (RAM) test. Methods: 40 female C57BL/6J mice in 3 months old were randomly divided into sham and ovariectomized (OVX) groups. OVX group was reassined to 3 subgroups(n=10) mice were ovariectomized and raised until 7 months old. 17β-estradiol time release pellet (0.18mg/pellet) was given ic in 7 days and 40 days before RAM were tested . Results: The percentage of errors in En...