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丝裂素活化蛋白激酶在AngⅡ上调培养的血管平滑肌细胞转化生长因子-β受体表达中的作用

The role of mitogen activated protein kinase in up-regulatio n of transforming growth factor receptor expression induced by angiotens Ⅱ in vascular smooth muscle cell in rats in vitro
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摘要 目的:探讨丝裂素活化蛋白激酶在血管紧张素Ⅱ(AngⅡ)诱导血管平滑肌细胞(VSMC)转化生长因子-β受体(TGF-βR1)上调中的作用。方法:培养大鼠主动脉VSMC,以10-7mol/L AngⅡ刺激作为AngⅡ组,AngⅡ刺激前分别应用10-5mol/L氯沙坦(Losartan)、PD98059预处理,以正常的VSMC为对照组,细胞免疫化学法测定培养12h时VSMC TGF-βR1的含量。结果:与对照组相比,AngⅡ刺激培养12h的VSMC TGF-βR1表达上调(P<0.01);AngⅡ受体AT1型拮抗剂Losartan使TGF-βR1表达显著降低(P<0.01),丝裂素活化蛋白激酶抑制剂PD98059能显著降低TGF-βR1表达(P<0.05)。结论:AngⅡ通过AT1上调TGF-βR1的表达,丝裂原活化蛋白激酶参与AngⅡ的胞内信号转导。 Objective:To study the role of mitogen activated protein kinase in up-regulation of transforming growth factor receptor induced by angiotensin Ⅱ in vascular smooth muscle cell in rats in vitro. Methods:The cultured VSMCs were Pretreated with 10-7mol/L AngⅡ as AngⅡ group, the cultured VSMC pretreated with Losartan, PD98059 with 10-7mol/L AngⅡ, the normol cultured VSMC as control group. TGF-βR1 was detected by immunocytochemical technique. Results :TGF-βR1 expression in AngⅡ group was higher than that in cont...
作者 孙银平 张大伟 王省 王晓冰 黄丽君
机构地区 新乡医学院病理生理教研室 新乡医学院解剖学教研室
出处 《陕西医学杂志》 CAS 北大核心 2008年第9期1128-1129,1173,共3页 Shaanxi Medical Journal
基金 河南省科技厅项目(424410129) 新乡医学院2006年高学历人才科研资助课题(2006010)
关键词 肌 平滑 血管/药物作用 血管紧张素Ⅱ 转化生长因子-β/代谢 Mulscle Smooth Vascular/drug effects Angiotensin Ⅱ Transforming growth factor beta/metabolism
分类号 R543 [医药卫生—心血管疾病]
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参考文献7

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二级参考文献45

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共引文献13

陕西医学杂志
2008年 第9期

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