肺癌患者胸腔积液及外周血CD_4^+CD_(25)^+T细胞、Foxp3基因表达及意义

Expression and significance of CD_4^+CD_(25)^+ T in pleural effusion and peripheral blood of patients with lung cancer

目的探讨CD4+CD2+5T细胞及Foxp3基因在肺癌发生、发展中的作用及机制。方法流式细胞仪检测32例肺癌合并胸腔积液患者外周血、胸腔积液及30例无胸腔积液肺癌患者外周血、胸腔冲洗液中CD4+CD2+5T细胞水平,RT-PCR法检测特异性转录因子Foxp3基因在胸腔积液及外周血中的表达。结果肺癌合并胸腔积液患者胸腔积液中CD4+CD25+T细胞水平显著高于无胸腔积液患者胸腔冲洗液及外周血;Foxp3基因在肺癌患者外周血和胸腔积液中呈高表达。结论肺癌患者外周血和胸腔积液中增高的CD4+CD2+5T细胞主要是CD4+CD2+5调节性T细胞。CD4+CD25+调节性T细胞可促进肺癌的进一步恶化,其机制可能是抑制肿瘤免疫。

Objective To explore the effect of the CD+ 4 CD+25 T and Foxp3 gene on the development of lung cancer.Methods The level of CD+ 4 CD+25 T lymphocytes in pleural effusion and peripheral blood from 32 patients with lung cancer with malignant effusion,pleural lavage and peripheral blood from 30 patients with lung cancer without effusion,and peripheral blood from 30 healthy control subjects were determined by flow cytometry.The expression of forkhead transcription factor Foxp3 was also examined.Results The level...