摘要
The new spreading conchospores of Porphyra yezoensis, P. haitanensis and P. katadai var. hemiphylla are induced by N-Methy-N’-Nitro-N-Nitrosoguanidine (NG), a ⑴strong mutagen to induce the mutant of chromatophore. It is showed that no mutants have ⑵been investigated in all control group. The conchospores of all the three species of Porphyra are easy to be induced by NG for they are in the stage of breeding, In the lowest dose (10 μg/mL) of this experiment, the mutant rate of P. yezoensis is up to 57.9%, that of P. haitanensis 30.7% and that of P. katadai var. hemiphylla ⑶51.7%. The mutant rate can not increase obviously by increasing the reagent concentration or prolonging the induction time. Within the scope of experiment, the inducement effects of three species of Porphyra are much similar, and the mutant rate is near 50%. The optimum induction concentration of NG is 10 μg/mL, and the optimum induction time is 30 minutes.
The new spreading conchospores of Porphyra yezoensis, P. haitanensis and P.katadai var. hemiphylla are induced by N-Methy-N′-Nitro-N-Nitrosoguanidine (NG), a strong mutagento induce the mutant of chromatophore. It is showed that (1) no mutants have been investigated inall control group. (2) The conchospores of all the three species of Porphyra are easy to be inducedby NG for they are in the stage of breeding, In the lowest dose (10 μg/mL) of this experiment, themutant rate of P. yezoensis is up to 57.9%, that of P. haitanensis 30.7% and that of P. katadai var.hemiphylla 51.7%. (3) The mutant rate can not increase obviously by increasing the reagentconcentration or prolonging the induction time. Within the scope of experiment, the inducementeffects of three species of Porphyra ar e much similar, and the mutant rate is near 50%. The optimuminduction concentration of NG is 10 μg/mL, and the optimum induction time is 30 minutes.
基金
Supported by NSFCC39770593
EMBLC CAS and Applied Fundamental Project of Jiangsu Province (BJ95182).
National Key Fundamental Research Program (G1999012009).
