铜绿假单胞菌感染模型中CC16 mRNA表达水平的研究

Clara cell secretory protein CC16 expression in rat model of pulmonary infection with Pseudomonas aeruginosa

目的 探讨铜绿假单胞菌感染模型中CC16mRNA及其蛋白水平与肺内炎症反应之间的关系。方法 铜绿假单胞菌经支气管接种制备小鼠感染模型,肺组织切片病理检查及支气管肺泡灌洗了解炎症反应程度,RT-PCR检测CC16mRNA表达水平,免疫组化染色检测肺组织CC16蛋白质水平。结果 接种细菌后24h肺内炎症反应最明显,CC16表达水平最低;至72h炎症反应减弱,CC16水平有所恢复。结论 CC16对铜绿假单胞菌感染所致炎症反应有抑制作用,同时亦可能对抗炎症反应所致肺损伤。

Objective To explore Clara cell secretory protein CC16 mRNA and protein levels in rat model of respiratory infection with Pseudomonas aeruginosa . Methods Intratracheal inoculation of 1 × 108 Pseudomonas aeruginosa to establish SD rat model of respiratory infection.Bronchoalveolar lavage and lung pathology were performed to observe inflammation in the lungs, while RT-PCR and histochem-istry staining were employed to detect CC16 mRNA expression and protein concentration.Results 24 hr after inoculation of Pseudomonas aeruginosa, neutrophils and macrophages infiltrated markedly in the lungs, while CC16 expression was lowest. At 72 hr CC16 expression level returned approximately to normal which was consistent with remission of inflammation. Conclusion CC16 might suppress lung inflammation caused by Pseudomonas aeruginosa infection, which is suggested to impair host capacity to clear bacterial,but protect lungs against inflammatory injury.