摘要
Objective To assess the relationship between murine recurrent spontaneous abortion and lymphocyte infiltration at the fetomaternal interface Materials & Methods Hysterolaparotomy was performed on d13. 5 of murine gestation, and resorption rate of embryos was calculated in two different mating combinations of abortion model CBA/J × DBA/2 and of the fertile control CBA/J × BALB/c,respectively. CD69 was used as an activation marker on NK cell surface, while DX5 was used as a common marker of NK cell in this study. The proportions of CD 69+ cells in isolated lymphocytes, and CD 69+ DX 5+ cells in DX5+ subpopulation at the fetomaternal interface were determined by two-color flow cytometry. It was further evaluated whether there was linear correlation between these cell proportions and resorption rate of embryos.Results Resorption rate of embryos were 36. 0% and 7. 2% (P ＜ 0. 01) in CBA/J ×DBA/2 and CBA/J × BALB/c (genotypes are all of H-2k × H-2d) , respectively. In CBA/J × DBA/2 mice, no linear correlation was observed between the proportion of CD69+ cells in lymphocytes (x) and resorption rate (y) (r= 0. 4054). However, it appeared that the proportion of CD69+DX 5+ cells/DX5+ cells (x) was strongly linear correlated with the resorption rate of embryos (y) (r = 0. 8156, y = - 0. 3958+2. 1237x) in this model.Conclusion Infiltration of CD 69+ DX5+ cells at the fetomaternal interface may be associated with recurrent embryo-resorption in CBA/J × DBA/2 mice.
基金
国家重点基础研究发展计划(973计划)，广东省广州市科研项目
