分子生物技术在酵母菌分类中的应用进展

The utilization of molecular biology in yeast taxonomy

随着生物技术的发展 ,一些新方法应用于酵母菌分类中 ,使酵母菌分类学研究从过去的传统分类系统过渡到以遗传特征为主要依据的现代分类系统。文章阐明了核磁共振氢谱、可溶性蛋白质凝胶电泳、脉冲电场凝胶电泳、REFP、RFLP和RAPD等 6种分子生物技术的方法、原理 ,以及在酵母菌分类鉴定方面的应用现状 。

Development of molecular biology leads to the rapid development of taxonomy of yeast from traditional taxonomy to modern systematic taxonomy based on genetic characteristics. This paper elucidates the basic concepts, principles of six methods covers: Proton Magnetic Resonance Spectroscopy(PMR), Soluble Protein gel Electrophorsis, PFGE, REFP, RFLP and RAPD, and their applications on taxonomy and identification of yeast Furthermore, the paper points out their limitations and application trends.