摘要
本试验用天然抗病毒剂VA作诱导因子 ,抗病品种枯斑三生烟 (NicotianatabacumL SamSunNN)和感病品种三生烟 (NicotianatabacumL SamSun)经VA诱导处理后 (清水作对照 )接种TMV。结果表明 ,VA可局部和系统地诱导烟草叶片对TMV产生抗性 ,其枯斑抑制率可达 4 0 %~ 6 0 % ,枯斑直径比对照减少 30 %~ 5 0 % ,且在抗病品种SamSunNN中 ,VA +TMV处理明显增加了PR蛋白的含量 ,在不接种情况下VA诱导的烟草叶片中PR蛋白表现很弱。在感病品种SamSun烟中 ,VA不能诱导PR蛋白产生。说明VA诱导PR蛋白含量的增加与VA抑制TMV在烟草体内的扩展有关。
VA,a natured virus inhibitor from several kinds of plants,was used as a elicitor.On the seventh day after treatment with VA,the newly developed leaves of Nicotiana tabacum L.SamSunNN and SamSun were inoculated with TMV.Result showed that localized and systemic resistance to TMV were induced in Nicotiana tabacum L.by VA.Local lesion inhibition rate was 40%-60%.Lical lesion diameter inhibition rate was 30%-50%.From 48 h to 72 h after treated with VA+TMV in SamSun NN tabacco plants,PR proteins were weaker in the TMV noninfected SamSun NN;Moreover,in SamSun tabacco plants,VA could not induce PR proteins with inoculation or noinoculation treatment.It indicated that VA could inhibite infection and replication of TMV.
出处
《华北农学报》
CSCD
北大核心
2002年第z1期50-55,共6页
Acta Agriculturae Boreali-Sinica
关键词
烟草
TMV
系统诱导抗性
局部诱导抗性
PR蛋白
抗病毒剂
Tobacco TMV
Systemic induced resistance
Localized induced resistance
PR proteins
Virus inhibitor