三株苏云金杆菌噬菌体的形态结构及其蛋白性质的比较研究

A Comparative Study on Morphology, Structure, and Protein Properties of Three Bacteriophages of Bacillus thuringiensis

对来自武汉微生物药厂的发酵裂解液中的噬斑形态、大小不同的 3株苏云金杆菌噬体的形态结构及其蛋白性质进行了比较研究。电镜观察发现 ,所有噬菌体的头部都呈长六棱柱状 ,具一短尾和一“衣领”状结构 ,并首次发现了“衣领”状结构是由 8～ 10个颗粒亚单位组成。 3株噬菌体所具有的这 8～ 10个颗粒亚单位对于噬菌体牢固地吸附于宿主表面应具有很强的促进作用 ,对于进一步研究噬菌体与宿主之间的关系提供一个结构上的证据。3株噬菌体的蛋白经SDS 聚丙烯酰胺凝胶电泳测定 ,都呈现 1条主带 ,分子量为 5 8884u ,1条次主带和 7条次带 ,表明 3株噬菌体的蛋白都是由 9种蛋白质构成。

Morphology, structure, and protein properties of three lytic bacteriophages of Bacillus thuringiensis from Wuhan Microbial Pesticides Factory with different shapes and sizes of plague were studied comparatively. It was found with electron microscope model JEM-100C that all phages posses long hexagonal prism on their heads, and a structure of short tails and a plate-like collar with 8～10 subunits attached to the base of the head. The 8～10 subunits have strong enhancement of adsorption of phages to the surface of the host, and provide structural evidence while studying the relationships between the hosts and the phages further. The three strains gave one major protein band, MW=58884d, one sub-major protein band, and seven sub-minor protein bands which were determined by SDS-polyacrylamide gel electrophoresis, and displayed that the protein of the three phages are composed of nine kinds of proteins.