适宜大豆灰斑病菌生长和产孢的培养基筛选试验

Screening Test on Nutrient Mediums Suited to Cercospora sojina Hara Growing and Spore-forming

将大豆灰斑病菌 (CercosporasojinaHara)在豆秸、豆荚、豆粒、豆粉、半综合、PDA、V8蔬菜汁 7种培养基上培养 ,测定了不同培养基对菌落生长速度和产孢量的影响。结果表明 ,豆粒、半综合、PDA、V8蔬菜汁培养基上的菌落生长速度比其他 3种快 ,每天平均生长 4 5 6 0～5 0 96mm2 ,但产孢量有很大差异。PDA和豆粒培养基产孢很少 ,平均每视野 2 4～ 4 2个 ,而半综合与V8蔬菜汁培养基的产孢量较大 ,为 2 8 4～ 30 2个。进一步对产孢量大的 2种培养基从原料成本和操作程序上比较 ,V8蔬菜汁比半综合培养基成本低 。

The colonial growing rate and spore_forming number of Cercospora sojina Hara are different on the seven kinds of nutrient mediums such as:soybean straw,soybean pod,soybean granule,soybean powder,semi synthetic medium,PDA and V8_juice mediums.The results showed that the colonial on soybean granule,semi synthetic PDA and V8_juice medium grow more quickly than the others.The average growth is 45.60～50.96 mm 2 every day,but the spore_forming number is different.The spore_forming number(28.4～30.2 spores/visual field)of the semi synthetic and V8_juice medium is greater than PDA and soybean granule medium(2.4～4.2 spores/visual field).The two kinds of mediums selected are compared in the cost and operating method.We concluded that not only the cost of V8_juice medium is cheap,but also the operating is easy too.