对成骨性肿瘤及瘤样病变bcl-2表达的分析

Analysis of the expression of bcl-2 in osteogenic tumor and tumor-diseases

目的 探讨 bcl- 2基因蛋白在成骨性肿瘤及瘤样病变中表达的方式和意义。方法 免疫组织化学染色 ,对骨肉瘤、骨化性纤维瘤、骨纤维结构不良及骨痂组织中 PCNA、p5 3、Fas和 bcl- 2蛋白检测 ,对其表达与肿瘤转移、组织形态学等指标进行比较分析。结果 PCNA和 p5 3在低分化骨肉瘤中呈高表达 ,bcl- 2在高分化骨肉瘤及骨化性纤维瘤中呈高表达。PCNA和 p5 3与 bcl- 2均高表达者肿瘤发生转移的机率较高 ,而仅 bcl- 2高表达者预后较好。结论 成骨性肿瘤中良性与高恶性的发病机制有所不同 :bcl- 2高表达导致异常细胞因生存期延长而过度积累可能是良性和低度恶性肿瘤的主要发病机制。

Objective To investingate the expression method of bcl-2 and it's significance in osteogenic tumor and tumor-like diseases.Method The protein of PCNA, p53, Fas and bcl-2 were examined immunohistologically in osteosarcoma, ossifying fibroma, fibeous dysplasia of bone and normal bone callus. The results were analysed combing with the information of metastasis and hisomorphology.Results PCNA and p53 were highly expressed in low-differentiated osteosarcoma, bcl-2 were highly expressed at high-differentiated osteocoma and ossifying fibroma. When PCNA, p53 and bcl-2 were all expressed at high level, the metastasis rate was high, when only bcl-2 was expressed at high level, the progmosis was better. Conclusion The mechanism for benign and malignant tumor are different, the benign and low-degree malignant tumor may be caused by over accumulated mutation cells sipported by high level of bcl-2, while malignant tumor may be the result of multi-gene cooperation abnormally.