兔阴茎海绵体平滑肌细胞的体外培养及其生物学特性

Biological Characteristics of Rabbit Corporal Smooth Muscle Cells Cultured in vitro

目的 :研究体外培养的新西兰白兔阴茎海绵体平滑肌细胞的生物学特性。 方法 :采用组织块培养法 ,对兔阴茎海绵体平滑肌细胞进行活细胞观察 ,并测定其细胞生长曲线、贴壁率、细胞分裂指数。 结果 :①兔阴茎海绵体平滑肌细胞为梭形 ,呈长轴平行排列 ,具有明显的方向性 ;②体外贴壁快 ,生长迅速 ,体外培养的阴茎海绵体平滑肌细胞在合适的传代条件和比例下能够生存并保持其稳定的生物学特性。 结论 :体外培养的兔阴茎海绵体平滑肌细胞模型可用于检测某些药物对阴茎勃起的影响。

Objectives: To study the biological characteristics of rabbit corporal smooth muscle cells(SMC) cultured in vitro . Methods: In vitro tissue culture technique, morphological observation, cell counting, mitosis index and adhesion rate evaluation were applied to study the biological features of the SMC. Results: ①SMC were spindle shaped and parallel along their longitudinal axis, showing obvious orientation. ②The attachment and the proliferation of SMC in vitro were rapid. SMC cultured in vitro can grow and maintain their steady characteristics provided appropriate passage rate and culture condition. Conclusions: The SMC cultured in vitro are proved to be used to evaluate and investigate the effect of some medicine on penile erection.