大鼠胚胎神经干细胞的培养和鉴定

The culture and identification of neural stem cell from embryonic rat

目的 体外培养和鉴定神经干细胞 .方法 从胎鼠前脑取出脑组织 ,采用酶消化、机械吹打、对倍稀释成单个细胞 ,经无血清培养获得单细胞克隆 ,由免疫细胞化学方法鉴定分离的神经干细胞 .结果 从胎鼠脑中分离的细胞具有连续传代形成克隆的能力 ,表达神经干细胞蛋白 (nestin) ,并能诱导分化成神经元和神经胶质细胞 .结论 分离的细胞是神经干细胞 .

Objective to culture and identify neural stem cells from embryonic rat. Method A cell line which has ability to form clones was isolated by using serum-free cell culture. Single cell technology was used to form sub-clones. Immunofluroscence was used to detect nestin antigen of the clone and specific antigen of neural cells after the clone differentiated. Results the clone cells express nestin antigen and has the ability to form sub-clones and differentiate into neurons, astrocytes and so on. Conclusion ...