赤芍承气汤对急性肝衰竭大鼠保护作用的实验研究

Experimental Study on Prevention of Chishao Chengqi Decoction on Acute Hepatic Failure in Rats

目的:探讨赤芍承气汤对内毒素脂多糖(LPS)致急性肝衰竭大鼠的保护作用。方法:以内毒素(LPS)加 D-氨基半乳糖(D-GalN)联合制备大鼠急性肝衰竭合并内毒素血症模型,以促肝细胞生成素颗粒剂(促肝康)和赤芍承气汤对模型动物进行治疗,观察血清 ALT、AST、TBIl 及肿瘤坏死因子-α(TNF-α)、内毒素(ET)的变化;常规 HE 染色,观察大鼠肝组织病理学改变;以免疫组化法检测 Fas、FasL 蛋白在大鼠肝组织中的表达情况,观察赤芍承气汤对肝细胞凋亡的作用。结果:LPS 加 D-GalN 可引起严重的急性肝坏死并有广泛的肝细胞凋亡。模型组 ALT、AST、TNF-α、ET 均显著升高,伴 Fas、FasL 蛋白在肝细胞中表达增强;中药组 ALT 及以上各指标的水平均较模型组明显降低(P<0.05),中药组大鼠肝坏死程度也较模型组明显减轻,肝细胞 Fas、FasL 阳性率,模型组为83%、92%,中药组为53%、50%,经统计学分析,差异有显著性(P<0.01)。促肝康可明显降低血清 ALT、AST,减轻肝组织病理损害。结论:赤芍承气汤能降低 ET、TNF-α的生成和释放,它和促肝康均具有良好的保护肝细胞、抗肝衰竭的作用;赤芍承气汤可减轻肝细胞 Fas/FasL 的表达,抵抗内毒素诱发的肝细胞凋亡。

Objective:To observe the effect of Chishao Chengqi Decoction(CSCQD)in preventing acute hepatic failure(AHF)induced by endo- toxin lipid polysaccharide(LPS).Methods:AHF complicated endotoxemia model was made with combined use of LPS and D-amine- galactose,and the model rats were treated with Cugankang and CSCQD separately.The changes of alanine transaminase(ALT),aspar- tate aminotransferase(AST),total bilirubin(TB),tumor necrosis factor α(TNF-α)and endotoxin(ET),as well as pathology of liver,Fas and FasL pro...