三七总皂甙对甲基硝基亚硝基胍转化的人胃粘膜上皮细胞GES-1原癌基因表达的影响

Effect of Penex Notogingseng Saponin on N-MethyI-N'-Nitro-N-Nitrosoguanidine Transformed Human Gastric Epithelial Cell Line GES-1 Oncogene Expression

目的:探讨三七总皂甙(PNS)对转化的人胃粘膜上皮细胞的影响。方法:免疫组织化学法检测 PNS 200 mg/L 对2×10^(-5) mol/L 甲基硝基亚硝基胍(MNNG)转化后的 GES-1细胞(MC 细胞)癌基因蛋白表达的影响,并以维甲酸(RA)10^(-5) mol/L 作为对照。结果:MC 细胞表皮生长因子受体(EGFR)表达与GES-1细胞比较无明显变化(P>0.05),但 C-erbB-2和 Bcl-2表达明显增加(P<0.05,P<0.01)。PNS 作用48 h 能明显降低 MC 细胞的 EGFR、C-erbB-2及 Bcl-2蛋白表达(与 MC 细胞比较,P<0.01)。RA 能显著降低 MC 细胞的 EGFR、C-erbB-2蛋白表达,但对 Bcl-2蛋白表达无明显影响。结论:MNNG 引起 GES-1细胞的转化与某些癌基因的表达增强有关,PNS 则可能通过抑制癌基因的表达来抑制转化细胞生长、促使转化细胞凋亡。

Objective:To study the effects of Penex notogingseng saponin(PNS)on transformed human gastric epithelial cells.Methods:Hav- ing been treated by 200 mg/L PNS,the oncogene protein expression in N-methyl-N -nitro-N-nitrosoguanidine(MNNG)transformed human gastric epithelial cell line GES-1(MC cell)was detected by immunohistochemical technique and compared with that effected by retinoic acid as control.Results:Immuno-reactive intensity of c-erbB-2 and bcl-2 expression were higher in MC cell than in untrans- form...