治糖保肾冲剂对早期糖尿病肾病大鼠肾皮质转化生长因子β_1表达的影响

Effect of Zhitang Baoshen Granule on Expression of Renal Cortex Transforming Growth Factor β_1 Gene with Early Diabetic Nephropathy Rats

目的:观察治糖保肾冲剂对早期糖尿病肾病(diabetic nephropathy,DN)大鼠肾皮质转化生长因子(transforming growth factor β_1,TGFβ_1)表达的影响。方法:48只 Wistar 大鼠,分为中药(治糖保肾冲剂)组、西药(苯那普利)组、模型组及空白组共4组,每组各12只。采用多次腹腔注射链脲佐菌素(streptozo-tocin,STZ)建立早期 DN 大鼠模型,喂药4周。运用逆转录聚合酶链反应(RT-PCR)和免疫组化法重点观察DN 大鼠肾皮质 TGFβ_1蛋白、基因表达的影响。结果:造模各组肾皮质 TGFβ_1 mRNA 明显高于空白组,以模型组最高,中药组最低。中、西药组与模型组比较,中、西药组组间比较差异均有显著性(P<0.01)。各组肾皮质 TGFβ_1免疫组化比较,肾小管表达强于肾小球,组间排列:模型>西药>中药>空白。结论:益气活血补肾的治糖保肾冲刺治疗 DN 机理之一是抑制早期 DN 肾皮质 TGFβ_1蛋白、基因的过度表达。

Objective:To observe the effect of Zhitang Baoshen Granule(ZTBS)on the expression of renal cortex transforming growth factor β_1 (TGFβ_1)gene with early diabetic nephropathy(EDN)rats.Methods:Forty-eight Wistar rats divided into 4 groups:Chinese medicine (ZTBS),western medicine(benazepril),model and normal groups,12 rats were in each gruop.EDN rat model was established by multi- ple abdominal injection of streptozotocin(STZ).After 4 weeks treatment,the effect on the expression of renal cortex TGFβ_1 protein ...