雷公藤多甙血清对肾小球系膜细胞凋亡及其调控基因的影响

Effects of Tripterygium Wilfordii Polyglycoside on Apoptosis and Apoptosis-Related Genes in Glomerular Mesangial Cells

目的:观察雷公藤多甙(TW)对肾小球系膜细胞增殖、凋亡、凋亡相关基因 ICE 和 bcl-2表达的影响。方法:采用肾小球系膜细胞培养方法进行体外研究,用不同剂量含雷公藤多甙的大鼠血清刺激4～6代系膜细胞16 h,MTT 法测定系膜细胞增殖指数:TUNEL 法定量观察凋亡细胞;琼脂糖凝胶电泳法观察凋亡细胞核小体 DNA 的断裂现象;逆转录-聚合酶链反应技术(RT-PCR)检测凋亡相关基因 ICE 和 bcl-2的表达变化。结果:加入雷公藤多甙血清16 h 后,系膜细胞出现了典型的凋亡征象,诸如细胞内深棕色颗粒形成、核固缩、碎裂和 DNA 梯形条带等;凋亡率显著高于空白血清组(P<0.01);同时 TW 各组 ICE 表达亦明显高于空白血清对照组,而抑制凋亡的 bcl-2基因各组均表达不明显。结论:雷公藤多甙可诱导系膜细胞凋亡,促进肾小球增殖性病变消散,并可能通过调控 ICE 和 bcl-2基因影响凋亡。

Objective:To observe the effects of Tripterygium wilfordii polyglycoside(TWP)on apoptosis and the expressions of apoptosis-relat- ed genes interleukin-1β-converting enzyme(ICE)and bcl-2 of glomerular mesangial cells in mice,so as to provide experimental evidence for regulating proliferation and apoptosis of mesangial cells with TWP.Methods:Four-six passaged mesangial cells were cultured with various concentrations of serum containing TWP stimulation for 16h.MTT assay was used to determine mesangial cells pr...