实验性脾虚证大鼠胃肠肽及回肠电—机械活动变化及加味四君子汤的影响

Changes of Gut Peptides and Electric Mechanical Activity of Ileum and Effect of Jiawei Sijunzi Decoction on Them in Experimental Rats with Spleen Deficiency Syndrome

目的:探讨加味四君子汤(简称中药)对实验性脾虚大鼠胃肠肽及回肠电-机械活动的影响。方法:采用放射免疫分析(RIA)方法对实验性脾虚证大鼠回肠始段、血液 P 物质(SP)、血管活性肠肽(VIP)进行测定,并用双银球电极-应变片记录了实验性脾虚证大鼠回肠始段的电-机械活动。结果:脾虚组 SP 含量显著升高(P<0.01),VIP 含量显著降低(回肠 P<0.05,血液 P<0.01),慢波和运动平均频率增高(P<0.05),运动振幅有增高趋势,快波明显增多;与脾虚组比较,自然恢复组 SP 含量明显恢复(回肠 P<0.01,血液 P<0.01),但未至正常;中药组 SP 含量降低(P<0.01),血液 VIP 含量显著回升(P<0.05),并接近正常,运动振幅显著下降(P<0.05),快波显著减少,其他指标无显著恢复。结论:脾虚证大鼠回肠始段 SP、VIP 含量改变可能与脾虚证大鼠回肠电-机械活动紊乱有关,中药对实验性脾虚证大鼠回肠始段胃肠肽的分泌及电-机械活动异常有一定的调理作用。

Objective:To study the effect of Jiawei Sijunzi Decoction(JSD)on gut peptides and electric mechanical activity of ileum in rats with Spleen Deficiency Syndrome(SDS).Methods:Radioimmunoassay(RIA)was used to measure the substance P(SP),vasoactive intestinal peptides(VIP),and double silver ball electrode stress plate was used to record the electric mechanical activity of the beginning segment of ileum.Results:In the rats with SDS,SP content raised significantly(P<0.01),VIP content lowered significantly(P<...