川芎嗪和灯盏花对慢性缺氧大鼠脑损伤保护作用的实验研究

Experimental Study on Protective Effect of Ligustrazine and Erigeron Breviscapus in Treating Chronic Hypoxia Induced Brain Damage in Rats

目的:探讨川芎嗪和灯盏花对慢性低氧脑的作用。方法:将 SD 大鼠分为正常对照组(A 组),低氧4周组(B 组),低氧4周加川芎嗪组(C 组),低氧4周加灯盏花组(D 组),先测取平均肺动脉压(mPAP)和平均颈动脉压(mCAP),再用生化方法测定,脑组织丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)变化,并用透射电镜方法,观察脑超微结构改变。结果:(1)B 组 mPAP 明显高于 A 组(P<0.01),C 组和 D 组低于 B 组(P<0.05),4组间 mCAP 差异无显著性。(2)B 组与 A 组比较,MDA 升高,GSH-Px、SOD 降低(P<0.05);C 组、D 组与 B 组比较,MDA 降低,GSH-Px、SOD 升高,但无统计学差异。(3) B 组毛细血管外水肿,神经元线粒体空泡变性,髓鞘分层松解,C 组、D 组神经元结构损害明显减轻。结论:慢性低氧对神经元线粒体及髓鞘改变与 MDA 升高有关,川芎嗪和灯盏花对慢性低氧脑损害有保护作用。

Objective:To explore the protective effect of ligustrazine(LTZ)and Erigeron breviscapus(EB)on chronic hypoxia induced brain damage.Methods:Forty rats were randomly divided into 4 groups:control group(group A),4 weeks of hypoxia group(group B),4 weeks of hypoxia and treated with LTZ group(group C),4 weeks of hypoxia and treated with EB group(group D).The mean pul- monary arterial pressure(mPAP)and mean carotid arterial pressure(mCAP)were determined at first,then the brain malondialdehyde (MDA),glutathion per...