局灶性脑缺血再灌注后脑组织Fos蛋白的表达与川芎嗪、黄芪对其表达的影响

Expression of Fos Protein in Rat Brains after Focal Cerebral Ischemia Followed by Reperfusion and Effects of Ligustrazine and Astragus

目的:探讨局灶性脑缺血再灌注后脑组织 Fos 蛋白的表达与川芎嗪、黄芪对其表达的影响。方法:采用免疫组化和医学图像分析相结合的方法,测定模型组、川芎嗪组、黄芪组及川芎嗪与黄芪合用组大鼠大脑皮层 Fos 蛋白阳性细胞的数目及平均灰度值。结果:(1)4组大鼠脑组织缺血侧 Fos 蛋白阳性细胞数目均多于缺血对侧,平均灰度值均低于对侧(P<0.01)。(2)在缺血侧脑组织,各药物干预组分别与模型组比较,Fos 蛋白阳性细胞数目均减少,平均灰度值均升高(P<0.01),其中川芎嗪与黄芪合用组 Fos 蛋白数目减少及平均灰度值升高更为显著(P<0.01)。结论:局灶性脑缺血再灌注后脑组织 Fos 蛋白表达显著升高。川芎嗪、黄芪以及两药合用均可部分抑制 Fos 蛋白的表达。两药合用比单用川芎嗪或黄芪下调 Fos 蛋白表达效果更显著,这可能是临床应用益气活血治则治疗缺血性中风的机理之一。

Objective:To investigate the expression of Fos protein in rat brains after focal cerebral ischemia followed by reperfusion and effects of ligustrazine(LZ),astragus.Methods:The immunohistochemistry and medical image analysis system were used to measure Fos positive cell number and mean grey level in rats cerebral cortex of model group,LZ group,astragus group,LZ and astragus group.Results:(1)In four groups,Fos positive cell number in ischemic sides was all higher than that in non-ischemic sides,mean grey lev...