高脂饮食对小鼠血脂蛋白氧化修饰反应及腹腔巨噬细胞集落刺激因子mRNA表达的影响和调肝导浊中药的调控作用

Effect of Cholesterol Rich Forage on Oxidized Low Density Lipoprotein and mRNA Expression of Macrophage Colony-Stimulating Factor and Regulating Function of Tiaogan Daozhuo Chinese Herbs

目的:观察高脂血清对小鼠血脂蛋白氧化修饰反应及巨噬细胞集落刺激因子(M-CSF)mRNA表达的影响,探讨调肝导浊中药防治高脂血症及动脉粥样硬化(AS)的作用机制。方法:分别以高脂饮食和中药灌喂小鼠12天,取血用 ELISA 法检测氧化低密度脂蛋白(OX-LDL)含量,用 Trizol 法提取总 RNA,RT-PCR 技术检测 M-CSFmRNA 表达。结果:调肝导浊中药对 M-CSF mRNA 表达有良好的正向调控作用,并抑制 OX-LDL 的生成。结论:调肝导浊中药可有效阻断 AS 形成、发展的关键环节。

Objective:.To observe the cholesterol rich forage on oxidized low density lipoprotein(OX-LDL)and mRNA expression of macrophage colony-stimulationg factor(M-CSF)and explore the mechanism of Tiaogan Daozhuo(TGDZ,liver regulating and turbid re- moving)herbs in preventing and treat ing hyperlipidemia and atherosclerosis(AS).Methods:The mice were fed with cholesterol rich for- are,in the feeding course they were controlled differently in groups for 12 days.After the animal experiment ended,the content of OX- LDL...