中药复方对老年痴呆模型小鼠学习记忆障碍及脑M受体含量的影响

Effect of Chinese Composite Recipe on Learning and Memory Disorder and Brain M-Receptor in Mice Model of Alzheimer's Disease

目的:观察中药复方对慢性铝中毒老年痴呆小鼠学习记忆障碍的保护作用,探讨其对 M 受体含量的影响。方法:小鼠60只随机分为6组,空白组和模型组用蒸馏水灌胃,脑复康组用脑复康液420 mg/(kg·d)灌胃,中药大、中、小剂量组分别用中药复方浓缩煎液34 g/(kg·d),17 g/(kg·d),8.5 g/(kg·d)灌胃,共55天。除空白组外,所有动物复制慢性铝中毒老年痴呆模型,并在末次给药30 min 后以跳台法进行学习训练,记录潜伏期及错误次数。24 h 后再测记忆成绩,并检测皮层及海马 M 受体亚型含量。结果:模型组记忆潜伏期明显缩短(P<0.01),学习潜伏期明显延长(P<0.01),学习和记忆的错误次数明显增多(P<0.01);用药各组记忆潜伏期均明显延长(P<0.01),学习潜伏期明显缩短(P<0.05或P<0.01)。中药3个剂量组学习和记忆错误次数亦明显减少(P<0.05),脑复康组记忆错误次数无明显改善(P>0.05);用药各组除中药小剂量组外均有提高模型小鼠皮层与海马区 M_1受体亚型含量的作用(P<0.05或 P<0.01),但对M_2受体亚型含量无明显影响。结论:中药复方对氯化铝所致小鼠学习记忆障碍具有保护作用,能明显提高老年痴呆模型小鼠皮层及海马区 M_1受体含量。

Objective:To observe the protective effect of Chinese composite recipe(CCR)on learning and memory disorder in model mice of Alzheimer s disease(AD)induced by aluminum chloride and to explore its effect on brain M-receptor content.Methods:Sixty mice were divided into six groups by random.Except for the control group,the Alzheimer s disease model was established in the other 5 groups by peritoneal injection of aluminum chloride.The 4 treated groups were fed by Naofukang liquid 420 mg/(kg·d),and concen- trated...