淫羊藿甙与葛根素对血管平滑肌细胞凋亡影响的比较研究

Comparative Study of Effect of Icarin and Puerarin on Vascular Smooth Muscle Cells Apoptosis

目的:比较不同浓度的淫羊藿甙和葛根素对血管平滑肌细胞(VSMC)凋亡的影响。方法:取家兔主动脉 VSMC 进行传代培养,第4代 VSMC 培养基中分别加入3个浓度的淫羊藿甙或葛根素,72h 后收集细胞,流式细胞仪测定细胞凋亡率,荧光显微镜观察凋亡细胞形态,透射电镜观察凋亡细胞超微结构。结果:各浓度淫羊藿甙和葛根素都可明显增高 VSMC 的凋亡率,并呈浓度依赖关系,荧光显微镜和透射电镜观察到典型的凋亡细胞形态学改变。但是低浓度淫羊藿甙引起细胞凋亡率高于同等浓度的葛根素,高、中浓度时两者间无明显差别。结论:淫羊藿甙、葛根素均有提高 VSMC 凋亡作用,低浓度淫羊藿甙作用似比低浓度葛根素强。

Objective:To compare the effects of icarin and puerarin on apoptosis of vessel smooth muscle cells(VSMC).Methods:In vitro,the smooth muscle cells derived from rabbit aorta were incubated with 3 dosages of icarin and puerarin for 72h.Flow cytometry and flouroscopy and transmission electron microscopy(TEM)was separately used to measure VSMC apoptosis ratio and morphological changes.Results:Flow cytometry proves that either icarin or puerarin in each doe,age results in a significant VSMC apoptosis ratio,and ap...