摘要
目的:观察川芎嗪对去氧肾上腺素(phenylcphrine,PHE)诱导大鼠胸主动脉收缩的阻断作用及机理。方法:用离体 SD 大鼠胸主动脉环作为实验对象,测定川芎嗪对其张力的影响。用细胞贴附式培养得到的单个平滑肌细胞,用标准的全细胞膜片钳技术记录大鼠平滑肌肌细胞膜 Cl通道的电流,观察不同浓度的川芎嗪对其影响。结果:川芎嗪对 PHE 引起动脉环双相收缩反应均有明显的阻断作用(P<0.01,n=8),其 IC_(50)值为3.2mmol/L;川芎嗪呈浓度依赖性的抑制100 mol/L KCl 的血管收缩效应,其 IC_(50)值为4.1mmo/L(P<0.01,n=6)。川芎嗪呈浓度依赖的抑制电压依赖性的 Cl^-通道电流-电压关系曲线(Ⅰ—Ⅴ)电流,但不影响其翻转电位。在测试电压为+100mV 时2.5、5mmol/L 川芎嗪对 Cl^-电流抑制率为45.45%、81.81%(P<0.01,n=6)。结论:川芎嗪对 PHE 诱导大鼠胸主动脉收缩有较好的松弛效应,其机理可能与影响 Ca^(2+)和 Cl^-通道跨膜转运有关。
Objective:To observe the blocking effect of tetramethylpyrazine(TMP)on phenylephrine(PHE)induccd rats thoracal aortic ring contraction and its mechanism on smooth muscle cells.Methods:Vascular contraction response was measured in isolated rat aortic ring, single smooth muscle calls of rat were obtained by cuhure method.The whole-cell patch clamp technique was used to record the change of chloride current following the admimistration of TMP.Results:TMP reduced biphasic contraction induced by PHE(P<0.01,n=...
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2002年第S1期1-3,共4页
Chinese Journal of Integrated Traditional and Western Medicine
关键词
川芎嗪
血管环
平滑肌细胞
CL^-通道
全细胞膜片钳技术
去氧肾上腺素
tetramethylpyrazine
aortic ring
smooth muscle cell
chloride channel
whole-cell patch clamp technique
phenylephrine
