In vitro-inhibition of shear-induced platelet aggregation by verapamil

1 ＩｎｔｒｏｄｕｃｔｉｏｎＲｅｃｅｎｔｅｖｉｄｅｎｃｅｓｕｇｇｅｓｔｓｔｈａｔｓｈｅａｒｉｎｄｕｃｅｄｐｌａｔｅｌｅｔａｇｇｒｅｇａｔｉｏｎ (ＳＩＰＡ)ｉｓａｎｉｍｍｐｏｒｔａｎｔｍｅｃｈａｎｉｓｍｏｆｔｈｒｏｍｂｏｓｉｓａｔａｒｔｅｒｉａｌｓｔｅｎｏｓｉｓ[2 ] .ＯｕｒｐｒｅｖｉｏｕｓｓｔｕｄｙｓｈｏｗｅｄｔｈａｔｓｏｍｅｏｆｔｈｅＣｈｉｎｅｓｅｈｅｒｂａｌｍａｔｅｒｉａｌｓｅｘｈｉｂｉｔｅｄｉｎｈｉｂｉｔｉｏｎｔｏＳＩＰＡ ,ｓｕｃｈａｓｌｉｇｕｓｔｒａｚｉｎｅ[6 ] .Ｏｕｒｓｔｕｄｙａｌｓｏｓｈｏｗｅｄｔｈａｔｌｉｇｕｓｔｒａｚｉｎｅｈａｄｅｆｆｅｃｔｏｆｉｎｈｉｂｉｔｉｎｇｉｎｔｒａｃｅｌｌｕｌａｒｃａｌｃｉｕｍｔｏｐｌａｔｅｌｅｔ[5 ] .Ｔｈｅｒｅｆｏｒｅ ,ｔｏｉｎｖｅｓｔｉｇａｔｅｔｈｅｅｆｆｅｃｔｏｆｖｅｒａｐａｍｉｌ,ａｔｙｐｉｃａｌｃａｌｃｉｕｍａｎｔａｇｏｎｉｓｔ,ｉｓｔｈｅｐｕｒｐｏｓｅｏｆｔｈｉｓｓｔｕｄｙ .2 Ｍａｔｅｒｉａｌｓａｎｄｍｅｔｈｏｄｓ2 1 ＡｎｉｍａｌｓＭａｌｅＮｅｗＺｅａｌａｎｄｒａｂｂｉｔｓｗｅｉｇｈｉｎｇ 2 .0— 2 .5ｋｇｗｅｒｅｓ...

The purpose of the study was to investigate the effect of verapamil on shear induced platelet aggregation(SIPA).Blood of New Zealand rabbit was collected to prepare platelet-rich-plasma (PRP) by routine centrifugation.A cone-plate shear device was employed to generate shear stress.The verapamil-treated PRP or control PRP were sheared at 37℃ for 120 sec.SIPA was determined from counting of tree platelet.The inhibition rates of SIPA by verapamil for five doses at three shear levels were calculated accordingly...