高效液相色谱法测定不同产地羌活中紫花前胡苷的含量

Determination of Content of Nodakenin in Notopterygium incisum Ting from Different Soure by HPLC

目的 :测定不同产地羌活中紫花前胡苷含量。方法 :高效液相色谱法。结果 :紫花前胡苷回归方程为Y =- 95 0 9.1+ 1912 70 9.8X ,r =0 .9998,线性范围 0 .16～ 0 .6 4 μg ,平均回收率 99.3% ,RSD2 .6 4 %。结论 :方法简单、快速。

Objective:To develop a method for the determination of nodakenin in N.incisum.Method:A HPLC method was used.Result:Y=-9509.1+1912709.8X,r=0.9998.The calibration was linear in range of 0.16～0.64μg.The average recovery was 99.7%,RSD 1.1%.Conclusion:The method is appropriate for the detemination of nodakenin.