龋病标本细菌培养结果分析

Analysis of anaerobic bacteria in the samples of decayed tooth disease

了解龋病中的厌氧菌群分布及形成机制,为口腔保健和龋病防治提供依据。选择50例龋病患者标本,采用厌氧培养箱,用抽气换气钯粒催化耗氧法,造成厌氧环境,孵育72～96h观察,并对不同可疑菌落,经耐氧试验后,对专性厌氧菌做生化反应鉴定到种。50例龋病标本经厌氧和需氧培养,阳性率100％。其中纯厌氧菌和纯需氧菌阳性者各占阳性标本的16.0％和8.0％,两者混合阳性占76.0％。共检出各种细菌110株。其中厌氧菌占60.0％,需氧菌占40.0％。龋病是在多种因素影响下,以厌氧菌为优势菌的多菌种混合感染所致。

To evaluate the distribution of the anaerobic bacteria flora and the mechanism of this flora sformation in the disease of decayed tooth, which can provide evidence of the oral cavity protection and the diseaseof decayed tooth of prevention and cure. Select the samples of 50 patients of decayed tooth, use anaerobiccultivation box, and draw oxygen and exchange oxygen Palladium granules catalysising consume oxygen method,to make a anaerobic environment, incubate 72～96h and observe, after the bearing oxygen tes...