氧化苦参碱抗丙型肝炎病毒的体外实验研究

The inhibitory effect of oxymatrine on hepatitis C virus in vitro

目的观察氧化苦参碱体外抗丙型肝炎病毒（ＨＣＶ）的活性。方法借助脂质体将重组ＨＣＶ基因（ｐＢＫ－ＨＣＶ）转染ＳＭＭＣ－７７２１细胞，并以此为模型，通过ｂＤＮＡ信号扩增法定量检测细胞内ＨＣＶ ＲＮＡ的变化，观察氧化苦参碱对ＨＣＶ ＲＮＡ的抑制作用； ＭＴＴ比色法观察药物的细胞毒性。结果建立了重组ＨＣＶ基因转染的稳定表达细胞模型，可作为药物研究的工具。氧化苦参碱浓度为１００— １０００μｇ／ｍｌ时能明显降低细胞内ＨＣＶＲＮＡ水平，有效浓度范围内无明显细胞毒性作用。结论氧化苦参碱能够在细胞水平有效地抑制ＨＣＶ ＲＮＡ，具有直接抗ＨＣＶ作用。

Objective To study the inhibitory effect of oxymatrine on HCV in vitro. Methods SMMC-7721 cells transfected with pBK-HCV using lipofectin transfection protocal were treated with oxymatrine. bDNA signal amplification assay and MTT colorimetric assay were used for intracellular HCV RNA and cytotoxicity. Results The stable HCV expression cell model established here could be used as a tool for medicine screen. Oxymatrine at the concentration of 100-1000 μg/ml could significantly decrease the level of intracell...