肿瘤坏死因子α对肝星状细胞表达结缔组织生长因子的影响

Effects of tumor necrosis factor a on the expression of connective tissue growth factor in hepatic stellate cells

目的研究肿瘤坏死因子（ＴＮＦ α）对肝星状细胞（ ＨＳＣ）结缔组织生长因子（ｃｏｎｎｅｃｔｉｖｅ ｔｉｓｓｕｅｇｒｏｗｔｈ ｆａｃｔｏｒ， ＣＴＧＦ）表达的影响。方法用链霉蛋白酶、胶原酶原位灌注， Ｎｙｃｏｄｅｎｚ一步密度梯度离心法分离正常大鼠 ＨＳＣ，并用 ＴＮＦ α，转化生长因子β １（ＴＧＦ β１）处理细胞；应用逆转录聚合酶链反应（ＲＴ—ＰＣＲ）技术分别观察不同培养时期的ＨＳＣ中的ＣＴＧＦ的表达。结果 ＴＮＦα， ＴＧＦβ１均诱导ＨＳＣ的ＣＴＧＦ表达； １０μｇ／Ｌ浓度的 ＴＮＦα作用 ６ｈ，没有观察到 ＨＳＣ表达 ＣＴＧＦ；作用 ２４ ｈ后，检测到有 ＣＴＧＦ ｍＲＮＡ的表达。 ＴＧＦ β１在 １μｇ／Ｌ浓度作用 ６ｈ后，即可诱导 ＨＳＣ的 ＣＴＧＦ ｍＲＮＡ表达。结论 ＴＮＦ α可诱导 ＨＳＣ的 ＣＴＧＦ ｍＲＮＡ表达，可能是其参与早期肝纤维化形成的作用机理之一。

Objective To investigate the effect of tumor necrosis factor α (TNF α ) on the expression of connective tissue growth factor (CTGF) in rat hepatic stellate cells (HSC) in vitro. Methods HSCs were prepared from SD rats by in situ perfusion and single-step Nycodenz gradient, and were treated with TNF α and TGF β 1. The expression of CTGF in HSC was assessed using reverse iran son ption-polymerase chain reaction (RT-PCR). Results Both TNFα and TGF β1 could stimulate the expression of CTGF mRNA in HSC. The exp...