TTV基因分型的研究

Genotypes of transfusion-transmitted virus

目的调查华南地区 ＴＴＶ感染流行情况和基因分型。方法选取 ＴＴＶ开放读码枢１的保守序列作内外引物， Ｇ１、 Ｇ２、 Ｇ３、 Ｇ４、 Ｇ５、 Ｇ６同源性极高的系列 ２ １６０— ２ １９６ ｎｔ作包被探针，异源性大于 ２０％的序列作显色探针Ｇ１、Ｇ２、Ｇ３、Ｇ４、Ｇ５、Ｇ６，采用ＰＣＲ微板核酸杂交ＥＬＩＳＡ技术对３８０例肝功能损害的肝炎患者血清进行ＴＴＶ检测及ＴＴＶ基因分型研究。结果检测出６１例ＴＴＶ阳性患者，检出率为１６．０％。对６１例ＴＴＶ进行基因分型，Ｇ１型４４例，Ｇ２型５例，Ｇ１、Ｇ２混合型感染１０例，Ｇ３、Ｇ４各１例，尚未发现Ｇ５、Ｇ６型。慢性肝炎中ＴＴＶ感染者与急性肝炎中ＴＴＶ感染者比例两者相近。结论在华南地区的肝炎疾病中， ＴＴＶ有较高的感染率； ＴＴＶ存在四个基因型，主要为Ｇ１型，其次为Ｇ２型，尚未发现Ｇ５、Ｇ６型。

Objective To investigate the genotypes of transfusion-transmitted virus (TTV) in South China. Methods One capture probe for all types of TTV from the conserved sequence nt2160 to nt2196 and six varied probes, heterogeneity more than 20% between each other for G1, G2, G3, G4, G5 and G6 type, respectively were synthesized. The primers coming from the conserved sequence of ORF1 were used to amplify TTV DNA from the serums of patients. Microplate sandwich hybridization-ELISA technique was used to detect the g...