摘要
研究了试剂3,6-二氯荧光素与蛋白质的显色反应,建立了一种测定蛋白质的新方法。在pH=7.00的缓冲溶液中,3,6-二氯荧光素与蛋白质发生灵敏的显色反应,生成1:1的复合物,最大吸收波长为500nm且在4h内稳定,蛋白质在3×10-8～3×10-7g/L范围内符合比尔定律,且回收率在90%～101%之间。探讨了氨基酸及金属离子K+等对测定的影响。测定了人血清中蛋白质的含量,结果满意。
The optimum conditions of complex reaction of a new chromogenic reagent dichlorofluorescein with protein were studied. The method was used in the photometric determination of protein. In a buffer medium of pH 7.00, dichlorofluorescein reacts with protein to form a complex (mole ration 1:1)having its absorption maxima at 500 nm, and the reactions steady with 4 hours, and recoveries are in the range of 90% to 101%. Beer's law is obeyed in the concentration in the range of 3×10^(-8)～3×10^(-7) g/L. The K^+ and others ion affect the reaction. The protein in human serum samples was determined, and the result is satisfactory.
基金
国家发展计划委员会高技术产业化示范项目(计高技[2000]1877)
