用LC/ESI-MS/MS研究肉苁蓉与其代用品中的苯乙醇苷类化合物

Analysis of Phenylethanoid Glycosides in the Extract of Herba Cistanchis by LC/ESI-MS/MS

目的 :研究正品肉苁蓉及其习用品盐生肉苁蓉和管花肉苁蓉中的苯乙醇苷类化合物的种类和含量。方法 :采用LC/ESI MS/MS方法对正品肉苁蓉及其习用品盐生肉苁蓉和管花肉苁蓉中的 7种苯乙醇苷类化合物进行定性分析及相对含量测定。结果 :正品肉苁蓉中鉴别出 7种苯乙醇苷类化合物 ;而盐生肉苁蓉中只含 6种 ;管花肉苁蓉中只含 5种 ,而且 3种肉苁蓉中苯乙醇苷含量也有差别。结论 :3种肉苁蓉中苯乙醇苷类化合物的种类和含量是区别各种肉苁蓉的特征成分之一。

AIM To analyze the phenylethanoid glycosides in Cis tanche deserticola Y.D.Ma and its alternatives. METHODS An HPLC/MS/MS method has been developed for the analysis of seven kinds of phenylethanoid glycosides in Cistanche deserticola Y.D.Ma, C.salsa (C.A.Mey) G.Beck and C.tubulosa (Schenk) R.Wight. The [M-H]^- ions were observed for five standards and Cistanche extracts. The glycosidic linkages, the core, and the attached sugar (s) of the phenylethanoid glycosides can be determined from the collision-indu...