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鞘氨醇调节人胰腺癌细胞株JF305生长和钙代谢 被引量：1

GROWTH REGULATING OF HUMAN PANCREAS CARCINOMA CELL JF305 BY SPHINGOSINE AND THE METABOLIZATION OF CALCIUM
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摘要 目的 :观察鞘氨醇对人胰腺癌细胞株 JF30 5的增殖作用 ;观察鞘氨醇对人胰腺细胞株 JF30 5内Ca2 + 浓度的影响 ,探讨鞘氨醇的细胞信使作用。方法 :细胞培养后 ,分别加入不同浓度的鞘氨醇 ,应用四甲基偶氮唑盐 MTT比色法来观察细胞增殖程度 ;应用 Fura- 2 / AM荧光比色测定 [Ca2 + ]i 结果 :鞘氨醇促进人胰腺癌细胞株JF30 5的生长 ,促进 JF30 5内 Ca2 +的生成 ,3min内达峰值 ,并在 0 .5 μ m ol/ L～ 2 .0 μ mol/ L 时呈剂量依赖性。结论 :鞘氨醇能促进人胰腺癌细胞株 JF30 5增殖 ;鞘氨醇的信使作用是通过刺激 Ca2 +转运的和 Ca2 Objective: To observe the growth promoting role of sphingosine (SPH) on human pancreas carcinoma cell strains JF305 and to study the role of sphingosine in signal transduction by measuring the concentrations of intracellular free calcium.Methods:JF305 cells were in medium,then were treated with SPH of different concentrations.Proliferation and viability of cell was measured by the Mosmann's method (MTT).Intracellular Ca 2+ level were measured using Fura 2/AM by a F 2000 flurorescence spectrophotomet...
作者 赵敏 王钢 姜若兰
机构地区 中国医科大学附属第一医院
出处 《肿瘤研究与临床》 CAS 2001年第3期152-154,共3页 Cancer Research and Clinic
关键词 鞘氨醇 [CA2+]I 人胰腺癌细胞株JF305 Human Pancreatic Cancer Cell Strains JF305 Sphingosine （SPH） Intracellular Free Calcium （Ca 2+ ）
分类号 R735.9 [医药卫生—肿瘤]
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参考文献8

  • 1Stevens V L,Nimkar S,Jarnison W C,et al.Characterization of the growth inhibition and cytotoxicity of long-chain(sphingoid)bases for chinese hataster ovary cells: evidence for an involvement of protein kinase C[].J Biochem Biophys Acte.1990
  • 2Ghosh T K,Bian J,Gill D L.Intracellular calcium release mediated by sphingosine derivatives generated in cells[].J Science.1990
  • 3Zhang H,Naishadl N D,Murphey M M,et al.Increases in phosphatidic acid levels accompany shingosine stimulated proliferation of quiescent Swiss 3T3 cells[].Journal of Biological Chemistry.1990
  • 4Carpio L C,Stephan E,Kamer A,et al.Sphingolipds stimulate cell growth via MAP kinase activation in osteoblastic cells[].Prostaglandins Leukot Essent Fatty Acids.1999
  • 5Jacobs LS,Kester M.Sphingolipids as mediators of effeects of plastelet -derived growth factor in vascular smooth muscle cells[].Journal of Applied Physiology.1993
  • 6Kimura T,Watanabe T,Sato K,et al.Sphingosine 1-phosphate stimulates proliferation and migration of human endothelial cells possibly through the lipid receptors, Edg-1 and Edg-3[].J Biochem J.2000
  • 7Zhang H,Buckley NE,Gibson K,et al.Sphingosine stimulates cellular proliferation via a protein kinase C-independent pathway[].Journal of Biological Chemistry.1990
  • 8Alessenko A V.The role of sphingomyelin in cycle metobolites in transduction of signals of cell proliferation, differentiation and death[].Membrane and Cell Biology.2000

同被引文献10

  • 1李柏林,梅慧生.燕麦叶片衰老与活性氧代谢的关系[J].植物生理学报（0257-4829),1989,15(1):6-12. 被引量：345
  • 2陈星,肖尊安,张崇浩.植物蛋白激酶C初探[J].植物生理学报（0257-4829),1996,22(4):437-440. 被引量：3
  • 3张志良.植物生理实验指导[M].高等教育出版社,1999.2:154-165.
  • 4MERRILL A H J R,SCHMELZ E M,DILLEHAY D L,et al.Sphingolipids-the enigmatic class lipid:biochemistry,physiology,and pathophysiology[J].Toxicol Appl Pharmacol,1997,142(1):208-251.
  • 5DBAIBO G S,WOLFF R A,OBEID L M,et al.Activation of a retinoblast oma protein dependent pathway by sphingosine[J].Biochem J,1995,310(2):453-459.
  • 6JARVIS W D,FORNARI F A J R,AUER K L,et al.Coordinate regulation of stress and mitogen activated protein kinases in the apoptotic actions of ceramide and sphingosine[J].Mol Pharmacol,1997,52(6):9352-9471.
  • 7PETRA SPERlING,EMST HEINZ.Plant sphingolipids:structural diversity,biosynthesis,first genes and functions[J].Biochimica et Biophysica Acta,2003,11-12.
  • 8ASADAK.Chloroplast:Formation of active oxygen and its scavenging[J].Methods Enzymol,1984,105:422-429.
  • 9谢芬,郭顺星.真菌来源鞘脂的结构及其活性研究进展[J].中国药学杂志,2002,37(7):481-484. 被引量：4
  • 10徐朗莱,叶茂炳.过氧化物酶活力连续记录测定法[J].南京农业大学学报,1989,12(3):82-83. 被引量：79

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肿瘤研究与临床
2001年 第3期

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