ELISA法检测人抗鼠抗体的研究

Screening the human antimouse antibodies with ELISA

目的 :对 10 0 0例健康人和 2 84例全身性红斑狼疮 (SL E)、类风湿性关节炎 (RA)、肾病、肿瘤患者中人抗鼠抗体 (HAMA)进行普筛 ,并对其产生情况进行初步探讨。 方法 :用酶联免疫吸附 (EL ISA)法检测待测血清中的HAMA。 结果 :10 0 0例正常健康人 HAMA阳性检出率为 1.1% ,76例 SL E、40例 RA、6 0例肾病、10 8例肿瘤疾病中 HAMA阳性检出率分别为 7.9%、5 .0 %、8.3%和 7.4%。 结论 :肿瘤患者的 HA MA阳性率与正常对照组相比差异显著 (P<0 .0 1) ,这可能是肿瘤患者较其他疾病组有较多机会接触鼠源性单抗治疗有关 ,其他疾患组总体阳性值未发现有统计学意义。

Objectives:To screen human anti mouse antibodies(HAMA) in sera from 284 patients with SLE, RA, tumors and nephropathy as well as 1000 healthy subjects and explore the conditions of their production. Methods:ELISA was used to detect HAMA in serum. Results:The positive rates of HAMA were 7.9%, 5.0 %,8.3% and 7.4% in sera from SLE, RA, tumors and nephropathy respectively while 1.1% in healthy subjects. Conclusions:A significant difference ( P <0.01) was found between tumor patients, those receiving mouse source monoclonal antibodies and other patient groups. There were no statistical differences of positive rates among other groups.