闽南肝癌高发区HBV-DNA荧光定量PCR检测的意义

Detecting Serum Quantitation of HBV-DNA with FQ-PCR Technique in A Area of Liver Cancer High-incidence, Southern-Fujian Area

[目的 ]分析闽南肝癌高发区乙肝病毒感染者血清 HBV- DNA含量与 HBVM的关系 ,探讨 HBV- DNA定量检测的应用价值。 [方法 ]用荧光定量 PCR技术 ,测定 398份血清中 HBV- DNA含量 ,同时检测 HBVM。 [结果 ]乙肝大三阳患者 HBV- DNA含量最高 (x=10 7.2 8± 1 .1 6 ) ,阳性率 10 0 % ,小三阳次之 (x=10 4.42± 1 .3 2 ) ,阳性率 5 4.8%。肝癌组HBV- DNA阳性率高达 6 6 .7% ,高于其他肝病组。 [结论 ]闽南肝癌高发区乙肝病毒感染人群中 ,HBV- DNA总阳性率较高 ,乙肝病毒持续复制可能是闽南地区肝癌发生的主要因素之一。

Objective] To study the relationship between HBV DNA quantitation and HBVM in sera of patients with hepatitis B in southern Fujian area and the application significance of HBV DNA detection [Methods] HBV DNA concentration and HBVM were detected by FQ PCR and ELISA respectively in 398 serum samples [Results] HBV DNA concentration and HBVM in 68 patients with HBsAg(+),HBeAg(+) and HBcAb(+) was 10 7 28±1 16 and positive rate was 100%;The concentration and HBVM were 10 4 42±1 32 and 54 8% in 177 patients with HBsAg(+),HBeAb(+) and HBcAb(+) The positive rate in liver cancers was 66 7% and higher than other chronic hepatitis groups [Conclusion] The positive rate of HBV DNA was at high level and it is suggested that the active reproduction of HBV may be one in important factor of high incidence liver cancer in southern Fujian area