耐热酵母菌株HU-TY-1的耐热机理初探

Study on heat shock protein and thermotolerant mechanism of S.cerevisiae

以酿酒酵母耐热菌株 HU- TY- 1和亲株 LK为材料 ,通过 SDS-聚丙烯酰胺凝胶电泳等技术 ,对它们在热休克应答前后的耐热性及蛋白图谱进行了分析 ,发现两个菌株经温和高温 (37℃ )诱导后可大幅提高对致死温度 (5 3℃ )的耐受力 ,同时伴随着分子量为 72 k D和 84 k D的热击蛋白 (HSP)的合成 ,其中耐热株 HU- TY- 1在常态时就有这两种热击蛋白的少量表达 .由此表明 :耐热酵母菌株 HU- TY- 1的高耐热性与 HSP72和 HSP84的组成性合成密切相关 .

The differences of protein synthesis and the survivors in high temperature between the thermotolerant S.cerevisiae stain HU-TY-1 and its primary stain LK have been compared by SDS-PAGE(sodium dodecanoyl sulphate-polyarcy lamide gel electrophoresis)and counting colonies. The results show that both the stains can synthesize HSP72 and HSP84 in heat shock response, and HU-TY-1 can synthesize these HSPs weakly at normal temperature. This suggests that the high thermotolerance of strain HU-TY-1 is relative tightly to the constitutive synthesis of HSP70 and HSP84.