期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

Apoptosis of matured T lymphocytes induced by mouse sertoli cells in cocultures in vitro

Apoptosis of matured T lymphocytes induced by mouse sertoli cells in cocultures in vitro
下载PDF
导出
摘要 Objective:To studywhethermousesertolicellscaninducetheapoptosisof maturedT lymphocytesincocultures in vitro.Methods:WithTUNEL,DNAelectrophoresis,electro-micrographyandflowcytometry,we examinedtheapopto-sisanditsratesof mousematuredT lymphocytesincontrolgroup(T lymphocytesonly),groupA(T lymphocytes+culture mediumof sertolicells),groupB(T lymphocytes+sertolicells).Results:Underelectro-micrography,chromatinconden-sation,karyopyknosis,karyorhexisandapoptoticbodywereobservedinsomeT lymphocytesin3groups;somenucleuses werestaineddarkbluewithTUNEL;a typicalDNAladderwas foundwithDNAelectrophoresis.Theapoptoticratesof T lymphocytesingroupA andB weresignificantlyhigherthanthatincontrolgroup(P<0.01).Theapoptoticrateof T lym-phocytesingroupB wassignificantlyhigherthanthatingroupA(P<0.01).Conclusion:Incocultureconditionin vitro,mousesertolicellscaninducetheapoptosisof maturedT lymphocytes. Objective:To studywhethermousesertolicellscaninducetheapoptosisof maturedT lymphocytesincocultures in vitro.Methods:WithTUNEL,DNAelectrophoresis,electro-micrographyandflowcytometry,we examinedtheapopto-sisanditsratesof mousematuredT lymphocytesincontrolgroup(T lymphocytesonly),groupA(T lymphocytes+culture mediumof sertolicells),groupB(T lymphocytes+sertolicells).Results:Underelectro-micrography,chromatinconden-sation,karyopyknosis,karyorhexisandapoptoticbodywereobservedinsomeT lymphocytesin3groups;somenucleuses werestaineddarkbluewithTUNEL;a typicalDNAladderwas foundwithDNAelectrophoresis.Theapoptoticratesof T lymphocytesingroupA andB weresignificantlyhigherthanthatincontrolgroup(P<0.01).Theapoptoticrateof T lym-phocytesingroupB wassignificantlyhigherthanthatingroupA(P<0.01).Conclusion:Incocultureconditionin vitro,mousesertolicellscaninducetheapoptosisof maturedT lymphocytes.
作者 刘阳 林子豪 朱晓海 刘善荣
出处 《Journal of Medical Colleges of PLA(China)》 CAS 2001年第3期183-185,共3页 中国人民解放军军医大学学报（英文版）
基金 SupportedbytheNationalNaturalScienceFoundationofChina(No.39870755)
关键词 SERTOLI CELL T LYMPHOCYTES APOPTOSIS sertolicell T lymphocytes apoptosis
分类号 R329.2 [医药卫生—人体解剖和组织胚胎学]
  • 相关文献

参考文献10

  • 1[1]Griffith TS, Brumer T, Fletcher SM et al. Fas ligand-induced apoptosis as a mechanism of immune privilege. Science, 1995;270 (5239): 1189
  • 2[2]Hunt JS, Vaasmer D,Ferguson IA et al. Fas ligand is positioned in mouse uterus and placenta to prevent trafficking of activated leukocytes between the mother and the conceptus.J Immunol, 1997; 158(9):4122
  • 3[3]Suda T, Takahashi T, Golstein P et al. Molecular cloning and expression of the fas ligand, a novel member of the tumor necrosis factor family. Cell, 1993; 75 (6): 1169
  • 4[4]Bellgrau D, Gold D, Selaury H et al. A role for CD95 ligand in preventing graft rejection. Nature, 1995; 377(19):630
  • 5[5]Perandones CE, Illera VA, Peckham D et al. Regulation ofapoptosis in vitro in mature murine spleen cells.J Immunol, 1993; 151(8):3521
  • 6[6]Vermes I, Haanen C, Steffens NH et al. A noval assay for apoptosis: Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labeled annexin V..J Immunol Methods, 7 1995; 184(1):39
  • 7[7]Suda T, Okazaki T, Naito Y et al. Expression of the fas ligand in cells of T cell lineage.J Immunol, 1995; 154(8):3806
  • 8[8]Alderson MR, Tough TW, Smith TD et al. Fas ligand mediates activation induced T cell death in human T lymphocytes.J Exp Med, 1995;181(1):71
  • 9[9]Sieg S, Smith D, Kaplan D. Differential activity of soluble versus cellular fas ligand: Regulation by an accessory molecule. Cell Immunol, 1999;195(2):89
  • 10[10]Allison J, Georgiou HM, Stresser A et al. Transgenic expression of CD95 ligand on islet β cells induces a granulocytic infiltration but does not confer immune privilege upon islet allografts. Pro Natl A cad Sci, 1997; 94(8):3943
Journal of Medical Colleges of PLA(China)
2001年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部