摘要
Objective:To observethedifferenceof geneexpressionsof braintissuesduringapoplecticepisodesandthose of normalbraininWistarratsinorderto studythepathologicalmechanismof apoplexy.Methods:A ratmodelof hyper-tensionwas establishedwiththeadministrationof coldstimulusandhighsaltintakeas theenvironmentalriskfactors.Apoplexyoccurredin theratsbecauseof hypertension.Suppressionsubtractivehybridization(SSH)was usedto identify andanalyzethedifferentialgenesspecificallyexpressedincerebraltissuesof stokegroupandcontrolrats.Re sults:A to-talof226genesoutof the228wereusableandanalyzed.Theaveragelengthof the226geneswas(286.6±120.3)bp with a rangefrom50bp to619bp.And126clonesoutof the226showeda sequencewithsignificantidentityto theknown genes;78clonesdemonstratedhomogenoussequencesto theexistingESTsof dbEST,butno oneof the78showedse-quencewithidentityto thatof knowngenes;andremaining22werenoveltransrciptsexhibitingno similarity to any knownsequences.Allthecloneswhichwerehighlyhomogenousto theknowngeneswerecategorizedon thebasisof their function.It was foundthat26.5%of themitochodrialgenesin braintissuesunderwentchangesafterapoplexyandthe changesshoweda twofoldrelationshipof causeandeffect.Conclusion:Environmentalfactorsareableto inducechanges of geneexpression,whichmayincreasethesensitivityto apoplecticstroke.
Objective:To observethedifferenceof geneexpressionsof braintissuesduringapoplecticepisodesandthose of normalbraininWistarratsinorderto studythepathologicalmechanismof apoplexy.Methods:A ratmodelof hyper-tensionwas establishedwiththeadministrationof coldstimulusandhighsaltintakeas theenvironmentalriskfactors.Apoplexyoccurredin theratsbecauseof hypertension.Suppressionsubtractivehybridization(SSH)was usedto identify andanalyzethedifferentialgenesspecificallyexpressedincerebraltissuesof stokegroupandcontrolrats.Re sults:A to-talof226genesoutof the228wereusableandanalyzed.Theaveragelengthof the226geneswas(286.6±120.3)bp with a rangefrom50bp to619bp.And126clonesoutof the226showeda sequencewithsignificantidentityto theknown genes;78clonesdemonstratedhomogenoussequencesto theexistingESTsof dbEST,butno oneof the78showedse-quencewithidentityto thatof knowngenes;andremaining22werenoveltransrciptsexhibitingno similarity to any knownsequences.Allthecloneswhichwerehighlyhomogenousto theknowngeneswerecategorizedon thebasisof their function.It was foundthat26.5%of themitochodrialgenesin braintissuesunderwentchangesafterapoplexyandthe changesshoweda twofoldrelationshipof causeandeffect.Conclusion:Environmentalfactorsareableto inducechanges of geneexpression,whichmayincreasethesensitivityto apoplecticstroke.
