Study with microarrays of the differential gene expression profiles of glioblastoma

Study with microarrays of the differential gene expression profiles of glioblastoma

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摘要 Objective:Thisstudyaimsto screenthedifferentiallyexpressedgenesof glioblastomausingmicroarraytech-nique.Methods:Specimensof glioblastomaandnormalbraintissuewereobtainedfrompathologicallyconfirmedpatients.A cDNAmicroarraycomprising14000clonescoveringthewholesetsof theretro-transcriptionalproductsof themRNAs of variousgliomasandthoseof normalbraintissueswas established,withwhichthedifferencesin geneexpressionbe-tweenglioblastomaandnormalbraintissueswereinvestigated.Results:Itwas foundthat94genesweremorethan3-fold differentiallyexpressedwith298morethandoubledintheglioblastomaincomparisonwiththenormalbraintissue.Some over-expressedgenesintheglioblastomawerescarcelyexpressedinnormalbraintissues,andseveralnovelgenesthatmay havebiologicalrelevanceintheprocessof gliomagenesiswereidentified.Conclusion:Microarraytechniquecombined withrelevantcDNArepositorycanfacilitaterapidlarge-scaleidentificationof potentialtargetgenesfordiagnosisandther-apy of glioma. Objective:Thisstudyaimsto screenthedifferentiallyexpressedgenesof glioblastomausingmicroarraytech-nique.Methods:Specimensof glioblastomaandnormalbraintissuewereobtainedfrompathologicallyconfirmedpatients.A cDNAmicroarraycomprising14000clonescoveringthewholesetsof theretro-transcriptionalproductsof themRNAs of variousgliomasandthoseof normalbraintissueswas established,withwhichthedifferencesin geneexpressionbe-tweenglioblastomaandnormalbraintissueswereinvestigated.Results:Itwas foundthat94genesweremorethan3-fold differentiallyexpressedwith298morethandoubledintheglioblastomaincomparisonwiththenormalbraintissue.Some over-expressedgenesintheglioblastomawerescarcelyexpressedinnormalbraintissues,andseveralnovelgenesthatmay havebiologicalrelevanceintheprocessof gliomagenesiswereidentified.Conclusion:Microarraytechniquecombined withrelevantcDNArepositorycanfacilitaterapidlarge-scaleidentificationof potentialtargetgenesfordiagnosisandther-apy of glioma.

作者杨志林徐如祥姜晓丹柯以铨罗成义金莹胡庚熙

出处《Journal of Medical Colleges of PLA(China)》 CAS 2001年第4期269-273,277,共6页 中国人民解放军军医大学学报（英文版）

关键词 GLIOMA MICROARRAY GENE expression glioma microarray geneexpression

分类号 R730.264 [医药卫生—肿瘤]

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Journal of Medical Colleges of PLA(China)

2001年第4期

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Study with microarrays of the differential gene expression profiles of glioblastoma

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