重型颅脑损伤后神经元细胞c-fos基因表达的双峰特征及机制研究

STUDY OF TWO-PEAK FEATURE OF c-fos EXPRESSION IN NEURONS AFTER SEVERE BRAIN INJURY AND ITS MECHANISM IN MICE

为探讨颅脑损伤时皮层神经元c fos基因表达的时相特征及机制 ,以小鼠重型颅脑损伤模型为对象 ,采用分子杂交和免疫组化等方法 ,对脑损伤后不同时间皮层神经元中c fos基因的表达状况进行检测。结果发现 ,颅脑损伤后皮层神经元中c fos基因表达的第一高峰在伤后 1h ,伤后 2 4h出现另一个表达高峰 ,c fos阳性表达神经元主要位于致伤侧大脑皮层ⅡⅣ层。提示颅脑损伤诱发的大脑皮层神经元c fos基因表达具有双峰的时相特征 ,其表达可能是由Leao播散性抑制引起 ,并与细胞内外信号转导与细胞凋亡有关。

To investigate the time dependent features and mechanisms of c fos expression in neurons after brain injury in mice. Using the mice model of severe traumatic brain injury, c fos expression in neurons was observed at different time points after trauma with the molecular hybridization and immunohistochemistry staining. A c fos expression process in neurons occurred after severe brain injury, peaking at 1 hour. Another expression peak appeared 24 hours later. Fos positive neurons were observed in the cerebral cortex of injured hemisphere, especially in Ⅱ Ⅳ layers. The c fos gene expression after brain injury in neuron has two peaks. c fos gene expression may be caused by Leao’s spreading depression, and may be associated with cell signal transduction and neuron apoptosis.